2PLR
Crystal structure of dTMP kinase (st1543) from Sulfolobus Tokodaii Strain7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-06-26 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.9789, 0.9793, 0.9000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.523, 62.873, 137.332 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.590 - 1.600 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pbr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.041 | 0.209 |
Number of reflections | 57129 | |
Completeness [%] | 99.4 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50% PEG 200, 100mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |