2PLO
D-(GTATACC) low temperature (100K)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.962 |
Spacegroup name | P 61 |
Unit cell lengths | 44.783, 44.783, 41.393 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.400 |
R-factor | 0.1773 |
Rwork | 0.170 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2pl8 |
RMSD bond length | 0.098 |
RMSD bond angle | 0.295 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.500 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.051 | 0.191 |
Number of reflections | 9382 | |
<I/σ(I)> | 20.2 | 3.9 |
Completeness [%] | 94.5 | 99.8 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 20 mg dissolved in 0.2 ml of 15 % MPD, 10-2 M sodium cacodylate buffer. Additives : 10-5M sodium azide, 10-2 M MgCl2 and 2.10-2 M spermine chloride, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | spermine chloride | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | sodium azide | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | spermine chloride | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | sodium cacodylate | ||
8 | 1 | 2 | sodium azide | ||
9 | 1 | 2 | MgCl2 |