2PLG
Crystal structure of T110839 protein from Synechococcus elongatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.98000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.185, 98.185, 144.679 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.540 - 2.600 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.23700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.100 | 0.630 |
Number of reflections | 26434 | |
<I/σ(I)> | 10 | |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 27.6 | 18.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 1.0 M Lithium sulfate, 0.5 M Ammonium sulfate, 0.1 M Tri-Na citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |