2PLC
PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM LISTERIA MONOCYTOGENES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-06-12 |
| Detector | SIEMENS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.500, 82.500, 92.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT AND SIG |
| Starting model (for MR) | 1ptd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.400 * |
| Data reduction software | XDS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 * | 0.250 |
| Total number of observations | 79316 * | |
| Number of reflections | 25523 | |
| <I/σ(I)> | 8.9 | 3.6 |
| Completeness [%] | 88.5 | 57.6 |
| Redundancy | 3.1 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | PROTEIN WAS CRYSTALLIZED FROM 0.1 M NA-HEPES PH 7.5, 0.8 M NA-K-TARTRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | reservoir | Na-Hepes | 0.1 (M) | |
| 3 | 1 | reservoir | Na/K tartrate | 0.8 (M) | |
| 4 | 1 | drop | precipitant |
