2PLB
D(GTATACC) under hydrostatic pressure of 1.39 GPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID27 |
Synchrotron site | ESRF |
Beamline | ID27 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.3738 |
Spacegroup name | P 61 |
Unit cell lengths | 42.830, 42.830, 40.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
R-factor | 0.1866 |
Rwork | 0.181 |
R-free | 0.22200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2pl8 |
RMSD bond length | 0.013 |
RMSD bond angle | 0.030 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.047 | 0.331 |
Number of reflections | 5518 | |
<I/σ(I)> | 11.4 | 2.2 |
Completeness [%] | 98.5 | 99 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 20 mg of DNA dissolved in 0.2 ml of 15% MPD solution cacodylate buffer 10-2 M. Additives: 10-5 M sodium azide, 10-2 M MgCl2, 2.10-2 M spermine chloride. Reservoir: same solution but 50 % MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 15% MPD | ||
10 | 1 | 2 | spermine chloride | ||
2 | 1 | 1 | cacodylate buffer | ||
3 | 1 | 1 | sodium azide | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | spermine chloride | ||
6 | 1 | 2 | 50% MPD | ||
7 | 1 | 2 | cacodylate buffer | ||
8 | 1 | 2 | sodium azide | ||
9 | 1 | 2 | MgCl2 |