2PLA
Crystal structure of human glycerol-3-phosphate dehydrogenase 1-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.79887 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.100, 139.100, 109.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.361 - 2.510 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x0v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.061 | 0.621 |
| Number of reflections | 42245 | |
| <I/σ(I)> | 19 | 3.39 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 50% PEG 300, 0.2M Sodium chloride, 0.1M Sodium potassium phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
