2PL6
Monoclinic crystal structure of hydrophobin HFBII in presence of a detergent
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-07-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.080, 66.410, 79.770 |
Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
Rwork | 0.224 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r2m |
RMSD bond length | 0.006 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.119 | 0.472 |
Number of reflections | 61949 | |
<I/σ(I)> | 7.7 | 3.1 |
Completeness [%] | 98.6 | 98.4 |
Redundancy | 2.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.48 | 293 | 0.1 M lithium sulphate, 20% polyethylene glycol (MW 2000), 0.1 M tris (pH 8.5), pH 8.48, VAPOR DIFFUSION, HANGING DROP, temperature 293K |