Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07217 |
| Spacegroup name | P 65 |
| Unit cell lengths | 102.645, 102.645, 62.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.540 |
| R-factor | 0.21029 |
| Rwork | 0.206 |
| R-free | 0.25284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | activated ecoli PhoP receiver domain structure without alpha helix 4 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.826 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 13785 | |
| <I/σ(I)> | 13.51 | 1.36 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 4.96 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 298 | precipitant: Sodium thiocyanate, Peg3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
