2PKV
D-(GGTATACC) ambient pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.3738 |
| Spacegroup name | P 61 |
| Unit cell lengths | 45.030, 45.030, 41.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18910 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 115D FROM PDB |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.330 |
| Number of reflections | 6228 | |
| <I/σ(I)> | 9.3 | 2.3 |
| Completeness [%] | 94.5 | 96.2 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 10 mg of lyophilized DNA dissolved in 0.2 ml of a 15 % MPD solution with additives: 10-5 M sodium azide, 10-2 M MgCl2 and 2.10-2 spermine chloride. Reservoir : same solution but 50 % MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium azide | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | spermine chloride | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | sodium azide | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | spermine chloride |
