2PKN
Crystal structure of M tuberculosis Adenosine Kinase complexed with AMP-PCP (non-hydrolyzable ATP analog)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5412 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.050, 75.184, 52.380 |
| Unit cell angles | 90.00, 109.80, 90.00 |
Refinement procedure
| Resolution | 57.540 - 1.900 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.25781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apo MTB ADK |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.275 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.540 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.050 |
| Number of reflections | 25991 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 20% PEG 8000, 100 mM sodium cacodylate pH 6.5, and 200 mM magnesium acetate tetrahydrate , VAPOR DIFFUSION, HANGING DROP, temperature 290K |
