2PK7
Crystal structure of the Q4KFT4_PSEF5 protein from Pseudomonas fluorescens. NESG target PlR1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97907, 0.97940, 0.96791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.415, 34.363, 35.832 |
| Unit cell angles | 76.39, 76.12, 73.58 |
Refinement procedure
| Resolution | 25.990 - 2.200 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.031 | 0.062 |
| Number of reflections | 11707 | |
| <I/σ(I)> | 25.5 | 12.6 |
| Completeness [%] | 88.4 | 83.9 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 24-28% PEG 3350, 0.2M Sodium chloride, 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
