2PJZ

The crystal structure of putative Cobalt transport ATP-binding protein (cbiO-2), ST1066

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9790
Spacegroup name	P 61 2 2
Unit cell lengths	102.094, 102.094, 104.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.800 - 1.900
R-factor	0.19821
Rwork	0.196
R-free	0.24172
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.549
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	25918
<I/σ(I)>	53.7	10.12
Completeness [%]	100.0	100
Redundancy	21.6	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.5M Ammonium sulfate, 0.1M Sodium citrate tribasic dihydrate, pH5.6, 1.0M Lithium sulfate monohydrate, pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K