2PJW

The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	95
Detector technology	CCD
Collection date	2006-07-12
Wavelength(s)	0.979493, 0.972425
Spacegroup name	P 32 2 1
Unit cell lengths	62.400, 62.400, 94.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.920 - 3.010
R-factor	0.222
Rwork	0.222
R-free	0.28500
Structure solution method	TWO WAVELENGTH MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.920	3.140
High resolution limit [Å]	3.010	3.010
Number of reflections	7506
<I/σ(I)>	18.1
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.85	288	1.1-1.4 M ammonium sulfate, 0.1 M Tris-HCl. Then micro-seeding into 1.25 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.85, VAPOR DIFFUSION, SITTING DROP, temperature 288K