2PJR
HELICASE PRODUCT COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.050, 62.600, 141.830 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| Rwork | 0.240 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.068 |
| Number of reflections | 31850 |
| Completeness [%] | 97.9 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | drop consists of 1:1 mixture of well and protein solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA substrate | (mM) | |
| 10 | 1 | reservoir | 50 (mM) | ||
| 11 | 1 | reservoir | PEG4000 | 12-20 (%) | |
| 2 | 1 | drop | glucose | (%) | |
| 3 | 1 | drop | (mM) | ||
| 4 | 1 | drop | ADPNP | (mM) | |
| 5 | 1 | drop | dithiothreitol | (mM) | |
| 6 | 1 | drop | Tris-HCl | (mM) | |
| 7 | 1 | drop | EDTA | (mM) | |
| 8 | 1 | drop | 106 (mM) | ||
| 9 | 1 | reservoir | Tris-HCl | 100 (mM) |
