2PI5
T7 RNA polymerase complexed with a phi10 promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 224.450, 73.791, 80.315 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.040 - 2.900 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 29104 |
<I/σ(I)> | 1.8 |
Completeness [%] | 96.6 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 50 mM Tris-HCl, 200 mM Li2SO4, 20% PEG 8000, 5% Glycerol, 15 mM magnesium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Tris-HCl | ||
10 | 1 | 2 | magnesium acetate | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | PEG 8000 | ||
4 | 1 | 1 | Glycerol | ||
5 | 1 | 1 | magnesium acetate | ||
6 | 1 | 2 | Tris-HCl | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | PEG 8000 | ||
9 | 1 | 2 | Glycerol |