2PGG
Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 122.103, 122.103, 359.017 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.970 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 | 0.338 |
| Number of reflections | 53819 | |
| <I/σ(I)> | 30.7 | 4.53 |
| Completeness [%] | 96.4 | 72.7 |
| Redundancy | 5.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 1.3M sodium malonate, 10mM sodium iodide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
