2PFQ
Manganese promotes catalysis in a DNA polymerase lambda-DNA crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-06-27 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.091, 63.485, 139.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.256 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsn |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.059 | 0.042 | 0.444 |
| Number of reflections | 26491 | ||
| <I/σ(I)> | 24.9 | ||
| Completeness [%] | 88.2 | 99.3 | 72.6 |
| Redundancy | 3.8 | 4.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 278 | 10-20% 2-propanol, 0.2 M sodium citrate and 0.1M sodium cacodylate , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 2-propanol | ||
| 2 | 1 | 1 | sodium citrate | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 2 | 2-propanol | ||
| 5 | 1 | 2 | sodium citrate | ||
| 6 | 1 | 2 | sodium cacodylate |
