2PD2
Crystal structure of (ST0148) conserved hypothetical from Sulfolobus Tokodaii Strain7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-19 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 3 |
Unit cell lengths | 112.272, 112.272, 112.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.500 - 2.060 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l1s |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.120 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.060 | 0.200 |
Number of reflections | 14722 | |
Completeness [%] | 98.3 | 82.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | OIL-BATCH | 8.5 | 293 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris-HCl, 30% PEG 4000, pH 8.5, OIL-BATCH, temperature 293K |