2PAG
Crystal structure of protein PSPTO_5518 from Pseudomonas syringae pv. tomato
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.424, 47.424, 122.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.710 - 1.600 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.044 |
Number of reflections | 18902 |
Completeness [%] | 98.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 400, 0.1 M Hepes, 0.2 M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |