2PAA
Crystal structure of phosphoglycerate kinase-2 bound to atp and 3pg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.854, 64.541, 75.936 |
| Unit cell angles | 90.34, 77.20, 85.03 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.197 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.405 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.056 | 0.125 |
| Number of reflections | 19197 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 96.9 | 91.7 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 30% PEG6000, 0.1 M bicine, 10 mM 3-D-phosphoglycerate, 5 mM ATP, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
