2P9Q
Crystal Structure of Phosphoglycerate Kinase-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-16 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.044, 80.850, 92.900 |
| Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjd |
| RMSD bond length | 0.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.056 | 0.244 |
| Number of reflections | 20415 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 95.9 | 92.8 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG4000, 0.2 M sodium acetate, 0.1 M Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.50 |
