2P99

E. coli methionine aminopeptidase monometalated with inhibitor YE6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.310, 61.700, 50.640
Unit cell angles	90.00, 105.43, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.227
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.359
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	19.140	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge	0.051	0.034	0.337
Total number of observations		2462	10812
Number of reflections	20706
<I/σ(I)>	10.5	15.3	2.2
Completeness [%]	97.9	97.7	95.9
Redundancy	3.7	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K