2P87

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-08
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	65.063, 65.063, 135.552
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.226
R-free	0.24900
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	0.274
Number of reflections	23240
<I/σ(I)>	18.4	3.6
Completeness [%]	92.6	96.2
Redundancy	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M Hepes pH7, 14% PEK4K, 0.2M MgCl2, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M Hepes pH7, 14% PEK4K, 0.2M MgCl2, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 298K