2P82

Cysteine protease ATG4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	94.408, 94.408, 337.339
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.690 - 2.100
R-factor	0.17679
Rwork	0.175
R-free	0.21279
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dli
RMSD bond length	0.011
RMSD bond angle	1.150
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	98033
<I/σ(I)>	31.86	2.7
Completeness [%]	95.2	68.1
Redundancy	7.6	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	291	Protein concentration 10 mg/mL. Reservoir: 23.5% PEG3350, 0.2M NaCl, 0.1M SPG buffer pH 5.4, 5% ethylene glycol. Cryoprotected in 20% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K