2P7Z
Estrogen Related Receptor Gamma in complex with 4-hydroxy-tamoxifen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | BRUKER SMART 6000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.990, 63.990, 137.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 2.500 |
| Rwork | 0.213 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ERRg BPA structure |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.158 |
| Data reduction software | SAINT |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 9739 | |
| <I/σ(I)> | 8.8 | 1.5 |
| Redundancy | 7.37 | 7.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 22.6% polyethylene glycol 4000, 0.1M Tris pH 8.5 and 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
