2P7N
Crystal structure of the Pathogenicity island 1 effector protein from Chromobacterium violaceum. Northeast Structural Genomics Consortium (NESGC) target CvR69.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97912, 0.97897, 0.96862 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 55.026, 66.981, 194.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.248 |
R-free | 0.29800 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.089 | 0.038 | 0.665 |
Number of reflections | 21495 | ||
<I/σ(I)> | 12.3 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 7.5 | 7.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 150 mM Sodium acetate pH 4.6, 22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 150 mM Sodium acetate pH 4.6, 22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 150 mM Sodium acetate pH 4.6, 22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |