2P6R
Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 138.379, 138.379, 252.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.232 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.089 | 0.168 |
| Number of reflections | 54172 | |
| <I/σ(I)> | 16.93 | 8.97 |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0; 22% MPD; 0.1M MgAcetate; 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MgAcetate | ||
| 4 | 1 | 1 | Glycerol | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | MgAcetate | ||
| 9 | 1 | 2 | Glycerol |
