2P6N

Human DEAD-box RNA helicase DDX41, helicase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-29
Detector	ADSC QUANTUM 315
Spacegroup name	P 65 2 2
Unit cell lengths	68.013, 68.013, 305.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.720 - 2.600
R-factor	0.24594
Rwork	0.243
R-free	0.29431
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i4i
RMSD bond length	0.016
RMSD bond angle	1.602
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.3.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.600	2.600
Rmerge	0.103	0.450
Number of reflections	13827
<I/σ(I)>	35.9	12.6
Completeness [%]	99.9	100
Redundancy	40	41.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	293	25% PEG 3350, 200mM Lithium sulfate, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.50