2P63
Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | SBC |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 61 |
| Unit cell lengths | 37.816, 37.816, 298.118 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.690 - 2.670 |
| R-factor | 0.22396 |
| Rwork | 0.220 |
| R-free | 0.27851 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.690 | 2.730 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.070 | 0.171 |
| Number of reflections | 6756 | |
| <I/σ(I)> | 21.15 | 8.47 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 9.81 | 6.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 50%MPD, 100 mM (NH4)H2PO4 ph 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
