2P5Q
Crystal structure of the poplar glutathione peroxidase 5 in the reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81560 |
| Spacegroup name | H 3 |
| Unit cell lengths | 221.665, 221.665, 48.142 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.178 |
| R-free | 0.20000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.067 | 0.409 |
| Number of reflections | 63667 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 5.04 | 4.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 0.05 M cadmium sulphate hydrate, 0.1 M HEPES, 1.0 M sodium acetate, pH 7.5, Microbatch, temperature 293K |
