2P51

Crystal structure of the S. pombe Pop2p deadenylation subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.8126
Spacegroup name	P 21 21 21
Unit cell lengths	52.370, 54.089, 90.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.470 - 1.400
R-factor	0.141
Rwork	0.139
R-free	0.20400
Structure solution method	SIRAS
RMSD bond length	0.013
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.470	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.052	0.636
Number of reflections	50856
<I/σ(I)>	26.7	1.98
Completeness [%]	99.6	99.7
Redundancy	4.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	18-24% PEG 8000, 200 mM Mg(CH3COO), 100-200 mM MES, pH 6.5, 0-10% glycerol, 5 mM BME, 1mM NaN3, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	18-24% PEG 8000, 200 mM Mg(CH3COO), 100-200 mM MES, pH 6.5, 0-10% glycerol, 5 mM BME, 1mM NaN3, VAPOR DIFFUSION, SITTING DROP, temperature 293K