2P4Q

Crystal Structure Analysis of Gnd1 in Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2005-11-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	147.261, 147.261, 114.428
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.430 - 2.370
R-factor	0.211
Rwork	0.211
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PGP.pdb
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	AUTOMAR
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.430	2.520
High resolution limit [Å]	2.370	2.370
Rmerge	0.084	0.363
Number of reflections	28662
<I/σ(I)>		1.7
Completeness [%]	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	1.28M tri-Sodium Citrate at pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K