2P4F

Crystal Structure of Atp11 functional domain from Candida Glabrata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-01-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97625
Spacegroup name	I 41
Unit cell lengths	63.187, 63.187, 153.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.400
R-factor	0.1449
Rwork	0.145
R-free	0.18620
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	0.031
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.058	0.476
Number of reflections	47858
<I/σ(I)>	16.1	1.59
Completeness [%]	81.2	15.3
Redundancy	10.3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG 400, MES, Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG 400, MES, Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K