2P4B
Crystal structure of E.coli RseB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.683, 197.681, 108.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.400 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 | 0.369 |
| Number of reflections | 39529 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 97.8 | 100 |
| Redundancy | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 6%(w/v) PEG 8000, 0.1M HEPES, 0.1M NaCl, 5%(v/v) MPD, 5%(w/v) guanidine-HCl, 3mM b-octylglucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
