2P48
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 2.3A resolution: SE5B-tetra crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.324, 42.324, 352.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.24611 |
| Rwork | 0.241 |
| R-free | 0.29651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p45 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 15006 |
| Completeness [%] | 94.9 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 295 | HAMPTON RESEARCH INDEX SCREEN, SOLUTION #66: (NH4)2SO4, PEG 3350, BIS-TRIS, PH 5.5, VAPOR DIFFUSION, temperature 295K |
