2P40
Crystal Structure of Dengue Methyltransferase in Complex with 7MeGpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2004-05-13 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.725, 114.725, 56.426 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.270 - 2.701 |
| R-factor | 0.20674 |
| Rwork | 0.204 |
| R-free | 0.25468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.433 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 12029 | |
| <I/σ(I)> | 21.7 | 5.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 0.4 M Ammonium Sulfate, 0.1 M Sodium Citrate, 1.2 M Lithium Sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
