2P38
Crystal Structure of Pyrococcus Abyssi Protein Homologue of Saccharomyces Cerevisiae NIP7P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.492, 90.284, 63.347 |
| Unit cell angles | 90.00, 134.29, 90.00 |
Refinement procedure
| Resolution | 31.670 - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.710 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.670 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 33002 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 4.1 M NACL, 100 MM HEPES, PH 7.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 7.20 |
