2P2T
Crystal structure of dynein light chain LC8 bound to residues 123-138 of intermediate chain IC74
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 44.356, 44.356, 204.466 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.180 - 3.000 |
| R-factor | 0.21797 |
| Rwork | 0.210 |
| R-free | 0.29634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.077 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 2817 | |
| <I/σ(I)> | 30 | 12.5 |
| Completeness [%] | 98.6 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
