2P2L
Rac1-GDP-Zinc Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X3A |
| Synchrotron site | NSLS |
| Beamline | X3A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-01-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.284 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.727, 89.727, 190.618 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.620 - 1.900 |
| R-factor | 0.17733 |
| Rwork | 0.176 |
| R-free | 0.20808 |
| Structure solution method | and molecular replacement |
| Starting model (for MR) | 1mh1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.627 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.620 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.512 |
| Number of reflections | 70803 | |
| <I/σ(I)> | 10 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100mM MES pH6.0, 15mM ZnSO4, 10% PEGMME 550., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
