2P2A
X-ray structure of the GluR2 ligand binding core (S1S2J) in complex with 2-Bn-tet-AMPA at 2.26A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-03-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0628 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.120, 121.980, 47.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.260 |
| Rwork | 0.211 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nnp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.085 | 0.180 |
| Number of reflections | 27218 | |
| <I/σ(I)> | 16.1 | 7.7 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 280 | PEG4000, Sodium Acetate, Ammonium Sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
