2P28

Structure of the PHE2 and PHE3 fragments of the integrin beta2 subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	52.260, 52.260, 423.924
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.263
Rwork	0.261
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yuk 1l3y
RMSD bond length	0.009
RMSD bond angle	1.223
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.257
High resolution limit [Å]	2.200	2.200
Number of reflections	18866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M ammonium sulphate, 15% PEG4000, 5% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K