2P1W
structure of the phosphothreonine lyase SpvC, the effector protein from Salmonella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-01-23 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 70.352, 70.352, 101.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.191 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE (2.12) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.361 |
| Number of reflections | 15052 | |
| <I/σ(I)> | 8.4 | 30 |
| Completeness [%] | 97.2 | 72.2 |
| Redundancy | 27.3 | 23.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12% PEG3350, 0.1M TrisHCl pH8.0, 5% Tacsimate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
