2P1R
Crystal structure of Salmonella typhimurium YegS, a putative lipid kinase homologous to eukaryotic sphingosine and diacylglycerol kinases.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.885596, 0.979239, 0.885596 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.000, 70.990, 102.160 |
| Unit cell angles | 90.00, 118.51, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| Rwork | 0.206 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.114 | 0.550 |
| Number of reflections | 45865 | |
| <I/σ(I)> | 14.46 | 1.74 |
| Completeness [%] | 97.6 | 83.6 |
| Redundancy | 6.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | pH 7.4, 17% PEG3350, 0.2M ammonium sulphate. VAPOR DIFFUSION, SITTING DROP, temperature 277K |
