2P1I
Plasmodium yoelii Ribonucleotide Reductase Subunit R2 (PY03671)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97972 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.040, 153.460, 143.360 |
Unit cell angles | 90.00, 132.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.700 |
R-factor | 0.30692 |
Rwork | 0.304 |
R-free | 0.36797 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1piy |
RMSD bond length | 0.022 |
RMSD bond angle | 2.004 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.767 |
Number of reflections | 104627 | |
<I/σ(I)> | 14.7 | 1.2 |
Completeness [%] | 98.7 | 88 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.0 M Sodium formate, 100 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |