2P1B

Crystal structure of human nucleophosmin-core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-23
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	99.262, 107.841, 108.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.750
R-factor	0.213
Rwork	0.213
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xe0
RMSD bond length	0.008
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.750	2.750
Number of reflections	31402
Completeness [%]	94.5	82.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	50MM AMMONIUM SULFATE, 50MM BIS-TRIS PH 6.5, 30%(W/V) PENTAERYTHRITOL ETHOXYLATE (15/4 EO/OH), pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 297K