2P0W
Human histone acetyltransferase 1 (HAT1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 116.557, 155.425, 53.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.350 - 1.900 |
| R-factor | 0.1846 |
| Rwork | 0.182 |
| R-free | 0.22834 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bob |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.350 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.100 |
| Number of reflections | 76880 |
| <I/σ(I)> | 8.6 |
| Completeness [%] | 99.0 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 12% PEG 20000, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
