2P0G
Crystal structure of Selenoprotein W-related protein from Vibrio cholerae. Northeast Structural Genomics target VcR75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.602, 81.602, 105.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.226 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oka |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO (1.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.100 | 0.050 | 0.386 |
| Number of reflections | 34831 | ||
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 99.7 | 98.7 | 99.7 |
| Redundancy | 6.9 | 7.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Na Acetate, 8% PEG 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
