2P0D

ArhGAP9 PH domain in complex with Ins(1,4,5)P3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9202
Spacegroup name	P 21 21 2
Unit cell lengths	41.510, 95.110, 29.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.570 - 1.811
R-factor	0.2
Rwork	0.199
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.982
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.048	0.361
Number of reflections	11480
<I/σ(I)>	17
Completeness [%]	99.2	93.6
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	26% PEG4000, 100mM HEPES, 10% glycerol, 5mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K