2P08
Structure of the N-terminally truncated PAS domain of signal transduction histidine kinase from Nostoc punctiforme PCC 73102 with homology to the H-NOXA/H-NOBA domain of the soluble guanylyl cyclase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-07 |
Detector | CUSTOM-MADE |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 72.332, 72.332, 169.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.360 - 2.000 |
R-factor | 0.24056 |
Rwork | 0.239 |
R-free | 0.27802 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.373 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.400 |
High resolution limit [Å] | 2.000 |
Number of reflections | 17432 |
<I/σ(I)> | 12.2 |
Completeness [%] | 99.4 |
Redundancy | 5.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 277 | 1.7-1.9M NH4SO4, 100mM TrisHCl pH 7.7-9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |