Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97600 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.301, 94.106, 117.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.520 - 1.210 |
| R-factor | 0.1008 |
| Rwork | 0.100 |
| R-free | 0.11946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qm4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.520 | 1.300 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.050 | 0.185 |
| Number of reflections | 113895 | |
| <I/σ(I)> | 23.09 | 10.93 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 7.16 | 6.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2M Lithium chloride, 0.1M Tris-HCl, 20% PEG 6000, 10.0% Ethylene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
